Apoptosis repressor with caspase recruitment domain (ARC) promotes bone regeneration of bone marrow-derived mesenchymal stem cells by activating Fgf-2/PI3K/Akt signaling

Abstract Objectives This study aims to investigate whether apoptosis repressor with caspase recruitment domain (ARC) could promote survival and enhance osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Materials methods The lentivirus transfection method was used establish ARC-overexpressing BMSCs. CCK-8 detect cell proliferation. BD Pharmingen™ APC Annexin V Apoptosis Detection kit apoptosis. capacity investigated by OCN immunofluorescence staining, ALP analysis, ARS assays, RT-PCR analysis. Cells were seeded into calcium phosphate cement (CPC) scaffolds then inserted subcutaneously nude mice the defect area rat calvarium. Histological analysis conducted evaluate in vivo new formation RNA-seq possible mechanism effect ARC on Results promoted BMSC proliferation inhibited enhanced vitro. An revealed that can inhibit increase formation. regulates BMSCs mainly activating Fgf-2/PI3K/Akt pathway. Conclusions present suggests is a powerful agent for promoting regeneration provides promising tissue engineering.